Department of Drug Science and Technology

Selected research products

$Marengo M., Fissore A., Oliaro-Bosso S., Adinolfi S. and Pastore A. Towards a metabolomic approach to investigate iron-sulphur cluster biogenesis. 2022 IUBMB LIFE-DOI: 10.1002/iub.2618. pp. 1-8

$Marengo M., Pezzilli D., Giacquinto E., Fissore A., Oliaro-Bosso S., Sgorbini B., Spyrakis F and Adinolfi S. Evaluation of porcine and aspergillus oryzae alpha amilases as possible model for human enzyme. 2022 PROCESSES; 10, 780. DOI.org/10.3390/pr10040780

Pippione A., Kovachka S., Sainas S., Rolando B., Denasio E., Adinolfi S., Zonari D., Lolli M., Spyrakis F., Oliaro-Bosso S. and Boschi D.New aldo-keto reductase 1C3 (AKR1C3) inhibitors based on the hydroxytriazole scaffold. 2022 Europ. J. if Med Chem; 237, 114366-84. DOI10.1016/j.ejmech.2022.114366

$Chirio D.; Peira E.; Sapino S.; Chindamo G.; Oliaro-bosso S.; Adinolfi S.; Dianzani C.; Baratta F.; Gallarate M. A new bevacizumab carrier for intravitreal administration: Focus on stability. 2021 FARMACEUTICS; 13, 560-577 DOI 10.3390/pharmaceutics13040560

Colletti A., Li S.,Marengo M., Adinolfi S.and Cravotto G. Recent advances and insights into bromelain processing, pharmacokinetics and therapeutic uses. 2021 APPLIED SCIENCES; 11,1-20.-DOI 10.3390/app11188428

Mauro M., Puglisi R., Oliaro-Bosso S., Pastore A. and Adinolfi S. Enzymatic and Chemical In Vitro Reconstitution of Iron-Sulfur Cluster Proteins. 2021 Chapter in the book “Fe-S Proteins. Methods and Protocols” page 79-95. DOI 10.1007/978-1-0716-1605-5_5

Ferrante T, Adinolfi S, D'Arrigo G, Poirier D, Daga M, Lolli ML, Balliano G, Spyrakis F, Oliaro-Bosso S. Multiple catalytic activities of human 17β-hydroxysteroid dehydrogenase type 7 respond differently to inhibitors. 2020 BIOCHIMIE; 170, 106-117. DOI 1016/j.biochi.2019.12.012

Lolli M L., Carnovale I. M., Pippione A. C., Wahlgren Y., Bonanni, D., Marini, E., Zonari, D., Gallicchio, M., Boscaro, V., Goyal P., Friemann R., Rolando B., Bagnati R., Adinolfi S., Oliaro-Bosso S. and  Boschi, D. Bioisosteres of Indomethacin as Inhibitors of Aldo-Keto Reductase 1C3. 2019. ACS MED. CHEM. LETTERS. 10, 437-443.  DOI 10.1021/acsmedchemlett.8b00484.

Willemse D., Weber B., Masino L., Warren R. M., Adinolfi S., Pastore A. and Williams M. J. Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. 2018. PloS One. 13, e0200145-30200172   DOI 10.1371/journal.pone.0200145.

Pippione A., Carnovale I., Bonanni D., Sini M., Goyal P., Marini E., Pors K., Adinolfi S., Zonari D., Festuccia C., Wahlgren W., Friemann R., Bagnati R., Boschi D., Oliaro-Bosso S., Lolli M. Potent and selective aldo-keto reductase 1C3 (AKR1C3) inhibitors based on the benzoisoxazole moiety: Application of a bioisosteric scaffold hopping approach to flufenamic acid. 2018. EUR. J. OF MED. CHEM. 150, 930-945. DOI 10.1016/j.ejmech.2018.03.040

*Adinolfi S, Puglisi R, Crack J, Iannuzzi C, Dal Piaz F, Konarev P, Svergun D, Martin S, Le Brun N and Pastore A. The molecular basis of the dual regulation of bacterial iron sulfur cluster biogenesis by CyaY and IscX. Front. Mol. Biosci. 2018, Feb2;4:97. doi: 10.3389/fmolb.2017.00097. eCollection 2017

• $ Puglisi R, Yan R, Adinolfi S, Pastore A. A new Tessera into the interactome of the isc Operon: A Novel interaction between HscB and IscS.

Front Mol Biosci. 2016, sept.27; 3:48. eCollection 2016.

• Vanocci T, Faggianelli N, Zaccagnino S, della Rosa I, Adinolfi S, Pastore A. A new cellular model to follow Friedreich's ataxia development in a time-resolved way. Dis. Model. Mech. 2015; 8:711-9.                 ISSN 1754-8403 1754-8411(web)

Impact factor 5.537   

Yan R, Adinolfi S, Pastore. Ferredoxin, in conjunction with NADPH and ferredoxin-NADP reductase, transfers electrons to the IscS/IscU complex to promote iron-sulfur cluster assembly. Biochim, Biophys. Acta 2015;1854:1113-7.

Pastore A and Adinolfi S. Chronochemistry in neurodegeneration. 2014 Frontiers in Molecular Neuroscience 2014: 31;7-20.

Yan R, Adinolfi S, Ianuzzi C, Kelly G, Oregioni A, Martin S, Pastore A. Cluster and fold stability of E. coli ISC-type ferredoxin. PLoS one 2013; 8:e78948

Yan R, Konarev PV, Iannuzzi C, Adinolfi S, Roche B, Kelly G, Simon L, Martin S R, Py B, Barras F, Svergun D I, Pastore A. Ferredoxin competes with bacterial frataxin in binding to the desulfurase IscS. Biol. Chem. 2013, 288:24777.

*Iannuzzi C, Adinolfi S, Howes BD, Garcia-Serres R, Clemancey M, Latour JM, Smoulevic G, Pastore A. The role of CyaY in iron sulfur cluster assembly on the E. coli IscU scaffold protein. PLoS One, 2011;6.   ISSN 1932-6203

Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A. Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat. Commun., 2010;1:95  

Smith LJ, Stapleton MR, Fullstone GJ, Crack JC, Thomson AJ, Le Brun NE, Hunt DM, Harvey E, Adinolfi S, Buxton RS, Green J. Mycobacterium tuberculosis WhiB1 is an essential DNA-binding protein with a nitric oxide-sensitive iron-sulfur cluster. Biochem J. 2010;432:417.

Prischi F., Pastore C, Carroni M, Ianuzzi C, Adinolfi S, Temussi P, Pastore A. Of the vulnerability of orphan complex proteins: the case study of the E. coli IscU and IscS proteins.

Prischi F, Giannini C, Adinolfi S, Pastore A. The N-terminus of mature human frataxin is intrinsically unfolded. FEBS J. 2009, 276: 6669-76.  

Adinolfi S, Iannuzzi C, Prischi F, Pastore C, Iametti S, Martin SR, Bonomi F, Pastore A.   Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Nat Struct Mol Biol. 2009; 6: 390. ISSN 1545-9993                                                             

Correia A, Ana R, Pastore C,Adinolfi S, Pastore P And Gomes C. M. Dynamics, stability and iron-binding activity of frataxin clinical mutants. FEBBS J. 2008;275:3680. 

Correia A, Adinolfi S, Pastore P And Gomes C M. Conformational stability of human frataxin and   effect of Friedreich's ataxia-related mutations on protein folding. Biochem J. 2006;398:605.

Pastore C, Adinolfi S, Huynen MA, Rybim V, Martin S and Pastore A. YfhJ, a molecular adaptor in iron-sulfur cluster formation or a frataxin-like protein? Structure 2006;14:1-14.

Ramos A, Hollingworth D, Adinolfi S, Castets M, Kelly G, Frekiel T A, Bardoni B, Pastore A. The structure of the N-terminal domain of the fragile X mental retardation protein: a platform for protein-protein interaction. Structure. 2006; 14:21.      

Zalfa F, Adinolfi S, Napoli I, Kuhn-Holsken E, Urlaub H, Achsel T, Pastore A, Bagni C. Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif. J. Biol. Chem. 2005; 280: 33403.   

de Chiara C, Menon RP, Adinolfi S, de Boer J, Ktistaki E, Kelly G, Calder L, Kioussis D, Pastore A. The AXH domain adopts alternative folds the solution structure of HBP1 AXH. Structure. 2005; 13: 743-53.   ISSN 0969-2126                                                                                      

Adinolfi S, Rizzo F, Masino L, Nair M, Martin SR, Pastore A. Bacterial IscU is a well folded and functional single domain protein. Eur J Biochem. 2004;271:2093.

Adinolfi S, Nair M, Politou A, Bayer E., Martin S Temussi P.and Pastore A. The factors governing the thermal stability of frataxin orthologues: how to increase a protein's stability. Biochemistry. 2004; 43:6511.  ISSN‎: ‎0006-2960 (print); 1520-4995 (web)

Bou-Abdallah F, Adinolfi S, Pastore A, Laue TM, Dennis Chasteen N. Iron binding and oxidation kinetics in frataxin CyaY of Escherichia coli. J Mol Biol 2004; 341:605-15.

Nair M, Adinolfi S, Pastore C, Kelly G, Temussi P, Pastore A. Solution structure of the bacterial frataxin ortholog, CyaY: mapping the iron binding sites. Structure. 2004; 12: 2037-48.      

Nair M, Adinolfi S, Kelly G, Frenkiel TA, Pastore A. NMR assignment of the 1H, 15N and 13C resonances of the E. coli frataxin orthologue, CyaY. J Biomol NMR. 2003; 27:403-4 

Adinolfi S, Ramos A, Martin SR, Dal Piaz F, Pucci P, Bardoni B, Mandel JL and Pastore A.The N-terminus of the fragile X mental retardation protein contains a novel domain involved in dimerization and RNA binding. Biochemistry. 2003; 42: 10437.

Bardoni B, Castets M, Huot ME, Schenck A, Adinolfi S, Corbin F, Pastore A, Khandjian EW, Mandel JL. 82-FIP, a novel FMRP (fragile X mental retardation protein) interacting protein,

de Chiara C, Giannini C, Adinolfi S, de Boer J, Guida S, Ramos A, Jodice C, Kioussis D, Pastore A. The AXH module: an independently folded domain common to ataxin-1 and HBP1. FEBS Lett. 2003; 551: 107.  ISNN 0014-5793 (print) 1873-3468 (web)     

Ramos A, Hollingworth D, Major SA, Adinolfi S, Kelly G, Muskett FW, Pastore A. Role of dimerization in KH/RNA complexes: the example of Nova KH3. Biochemistry. 2002;41:4193.

39) Adinolfi S, Trifuoggi M, Politou AS, Martin S and  Pastore A. A structural approach to understanding the iron-binding properties of phylogenetically different frataxins. Hum Mol Genet. 2002; 11: 1865.

Musco G, Stier G, Kolmerer B, Adinolfi S, Martin S, Frenkiel T, Gibson T, Pastore  A. Towards a structural understanding of Friedreich's ataxia: the solution structure of frataxin. Structure 2000; 8: 695. 

Musco G, de Tommasi T, Stier G, Kolmerer B, Bottomley M, Adinolfi S, Muskett FW, Gibson TJ, Frenkiel TA, Pastore A. Assignment of the 1H, 15N, and 13C resonances of the C-terminal domain of frataxin, the protein responsible for Friedreich ataxia. J Biomol NMR. 1999; 15: 87-8       

Adinolfi S, Bagni C, Musco G, Mazzarella L and Pastore A. Dissecting FMR1, the protein responsible for fragile X syndrome, in its structural and functional domains.  RNA 1999; 8: 1248.  

Adinolfi S., Bagni C., Castiglione Morelli M. A., Fraternali F., Musco G. and Pastore A. Novel RNA-binding motif: the KH module. Biopolymers 1999; 51, 153.   

Vitagliano L, Adinolfi S, Sica F, Merlino A, Zagari A and Mazzarella L. A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease.  J. Mol. Biol. 1999; 293: 569.

Vitagliano L, Adinolfi S, Riccio A, Sica F, Zagari A, Mazzarella L. Binding of a substrate analog to a domain swapping protein: X-ray structure of the complex of bovine seminal ribonuclease with uridylyl(2',5')adenosine.  Protein Science, 1998; 7; 1691.  ISSN 0961-8368 (print) 1469-896X (web)

Adinolfi S, Piccoli R, Sica F, Mazzarella L. BS-RNase tetramers: an example of domain-swapped oligomers. FEBS Letter, 1996; 398: 326-332  

Courses

• Approccio strutturale per la comprensione delle malattie monogeniche (STF0295)
  Corsi di studio in
  Farmacia - Chimica e tecnologia farmaceutiche
• BASI MOLECOLARI DELLE MALATTIE GENETICHE E DELLE VIE DI TRASDUZIONE DEL SEGNALE CELLULARE (STF0265)
  Corsi di studio in
  Farmacia - Chimica e tecnologia farmaceutiche
• Basi molecolari e genetiche delle malattie (STF0035)
  Corsi di studio in
  Farmacia - Chimica e tecnologia farmaceutiche
• Biochimica applicata (CTF) (FAR0025 )
  Corsi di studio in
  Farmacia - Chimica e tecnologia farmaceutiche
• Biochimica applicata medica - Farmacia (STF0050)
  Corsi di studio in
  Farmacia - Chimica e tecnologia farmaceutiche
• Computer-aided drug design (STF0218)
  Corsi di studio in
  Farmacia - Chimica e tecnologia farmaceutiche

Research topics

Professor Adinolfi is interested in the study of the structure-function 
relationship of proteins important for the pathogenicity of microorganisms, 
involved in neurodegeneration and deriving from natural sources

Research groups

• Biochemistry and Molecular Biology (BMB)

Research projects

• Characterization of Annexin A11: a new protein associated with ALS
• Characterization of Fe-S cluster biogenesi in Mycobacterium tuberculosis
• CRPCcare

Activities in agenda

See my calendar

Academic bodies

• Consiglio di Dipartimento
• Giunta

Office hours